![]() Various further developments have emerged in automated scoring ( 6– 8) and the cooperation in international networks ( 9). In this context, the conventional and established methods of biological dosimetry, such as the analysis and quantification of dicentric chromosomes, translocations or micronuclei, will continue to play a central role in the future ( 4, 5). on future applications in the field of emergency dosimetry, molecular epidemiological studies, personalized dosimetry after medical exposures or in the emerging sector of space tourism ( 3). This was highlighted in a recent review paper by Ainsbury et al. However, biological dosimetry includes much more than the conventional investigation of a single individual for possible overexposure to ionizing radiation. Thereby, the classification of the exposure level is based on established dose-response curves, which are derived from the analysis of blood samples irradiated in vitro under known conditions ( 2). Suspected overexposure to ionizing radiation can be detected and quantified in biological dosimetry using specific biomarkers in peripheral blood ( 1). Careful consideration of the experimental setup in collaboration with physicists is required to ensure traceability and reproducibility of irradiation conditions, to correlate the radiation dose and the number of aberrations correctly and to avoid systematical bias influencing the dose estimation in the frame of biological dosimetry. Therefore, dosimetric monitoring of experimental irradiation setups is mandatory prior to the establishment of calibration curves in biological dosimetry. An additional fan heater had no consistent impact. The number of dicentric chromosomes and micronuclei differed by ~30% between both orientations. horizontal) had a significant effect on the radiation dose with a variation of −41% up to +49% and contributed to a dose gradient of up to 870 mGy inside the vertical tubes due to the size of the sample tubes and the associated differences in the distance to the focal point of the tube. This study revealed that the orientation of the sample tubes (vertical vs. To evaluate the influence of the setups, physical dose measurements using thermoluminescence dosimeters, electron paramagnetic resonance dosimetry and ionization chamber as well as biological effects, quantified by dicentric chromosomes and micronuclei, were compared. Blood collection tubes were irradiated with a dose of 1 Gy in vertical or horizontal orientation in the center of the beam area with or without usage of an additional fan heater. ![]() In this study, irradiation was performed with an X-ray source (195 kV, 10 mA, 0.5 mm Cu filter, dose rate 0.52 Gy/min, 1 st and 2 nd half-value layer = 1.01 and 1.76 mm Cu, respectively, average energy 86.9 keV). The aim of this study was to investigate variations and pitfalls associated with the experimental setups used to establish calibration curves in biological dosimetry with X-ray cabinets. Accurate physical dosimetry of the irradiation performance is a critical part of the experimental procedure and is influenced by the experimental setup, especially when X-ray cabinets are used. Therefore, blood samples are irradiated in vitro and evaluated based on the applied assay. In biological dosimetry, dose-response curves are essential for reliable retrospective dose estimation of individual exposure in case of a radiation accident. 3Department of External Dosimetry, Institut de Radioprotection et de Sûreté Nucléaire (IRSN), Fontenay-aux-Roses, France. ![]() ![]()
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